Internationally Recognized Expert in Stem Cell Therapy

Equine stem cells can be isolated and culture expanded in 14 days or less.


Stem Cell Therapy

Dr. Hackett completed her PhD developing techniques in the isolation, characterization, and efficient culture expansion of equine Mesenchymal Stem Cells. She is an internationally recognized expert in the field of stem cell therapy. In her new state-of-the-art laboratory, equine stem cells can be isolated and culture expanded in 14 days or less into therapeutic doses for treatment of tendinitis or chronic arthritis.


How do stem cells work?

Current research and clinical trials in human and veterinary medicine suggest that the main effect of stem cell therapy is modification of the healing environment through growth factor release and modulation of the inflammatory response at the site of injury. Stem cells function to reduce scar tissue formation and promote superior healing compared to injuries treated with similar protocols without the addition of stem cell therapy. To date, most of the clinical use of MSC in the horse has focused on tendonitis and joint disease, but potential beneficial applications for other inflammatory/immune conditions are being explored in both basic science and clinical research trials.


Dr. Hackett can assist you with all or a portion of the following:

  • Sternal bone marrow aspiration Frontal view of a bone marrow aspiration taken from the sternum of an adult horse under standing sedation. Please note minimal pressure is needed to obtain an aspirate when the needle is fully seated in the marrow cavity. Also, the location of the needle on midline is very important. The pectoral muscles can be used as a guide for left to right needle placement on midline. Ultrasound can be used to guide needle placement in the front to back plane to minimize risk during entry into the marrow space.
  • Stem cell isolation and culture expansion Centrifugal separation of the bone marrow aspirate over a density gradient solution is an effective method to rapidly enrich the MSC forming cells compared to raw bone marrow. Over 99.9% of red blood cells are removed prior to culture using this technique, maximizing efficiency. In the photo, dilute aspirate (red) is carefully layered over the sugar solution (clear) at the bottom of the tube before separation.
  • Ultrasound guided implantation Ultrasound can be used to guide MSC injection directly into the core lesion (inner area marked by an X) of a damaged tendon (outer circle marked by the +). Although direct injection provides immediate MSC concentrations at the site of injury, IV regional limb perfusion of MSCs can be considered in locations inaccessible to injection or in patients where multiple locations in the same limb could benefit from cell therapy.

About Primus Equine

"I completed my PhD in developing techniques in the isolation, characterization, and efficient culture expansion of equine Mesenchymal Stem Cells."

Dr. Catherine H. Hackett